› 4afe

X-ray diffraction
2.6Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase Nek2 Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 32.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P51955 (Residues: 1-271; Coverage: 61%)
Gene names: NEK2, NEK2A, NLK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GGY 1 x GGY
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 101.039Å b: 56.724Å c: 74.279Å
α: 90° β: 127.1° γ: 90°
R-values:
R R work R free
0.218 0.216 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Cell cycle regulation by the NEK family of protein kinases.
Fry et al. (2012)
8 more

4 mentions without citation

Nek2 Kinase Signaling in Malaria, Bone, Immune and Kidney Disorders to Metastatic Cancers and Drug Resistance: Progress on Nek2 Inhibitor Development.
Dana et al. (2022)
3 more