› 4agi

X-ray diffraction
1.6Å resolution

Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) in complex with seleno fucoside.

Released:
DOI: 10.2210/pdb4agi/pdb
PDB entry 4agi coloured by chain, front view.
PDB entry 4agi coloured by chain, side view.
PDB entry 4agi coloured by chain, top view.
Source organism: Aspergillus fumigatus
Primary publication:
A soluble fucose-specific lectin from Aspergillus fumigatus conidia--structure, specificity and possible role in fungal pathogenicity.
Houser J, Komarek J, Kostlanova N, Cioci G, Varrot A, Kerr SC, Lahmann M, Balloy V, Fahy JV, Chignard M, Imberty A, Wimmerova M
PLoS One 8 e83077 (2013)
PMID: 24340081

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fucose-specific lectin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 315 amino acids
Theoretical weight: 34.74 KDa
Source organism: Aspergillus fumigatus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q4WW81 (Residues: 1-315; Coverage: 100%)
Gene names: AFUA_5G14740, fleA
Sequence domains: Fungal fucose-specific lectin
Structure domains: Fucose-specific lectin

Ligands and Environments

1 bound ligand:
Ligand SFU 26 x SFU
1 modified residue:
Ligand CSD 4 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P1
Unit cell:
a: 46.691Å b: 79.847Å c: 84.389Å
α: 91.09° β: 88.89° γ: 103°
R-values:
R R work R free
0.154 0.152 0.185
Expression system: Escherichia coli BL21

Quick links

Citations

16 review citations

Interactions of Aspergillus fumigatus Conidia with Airway Epithelial Cells: A Critical Review.
Croft et al. (2016)
15 more

6 mentions without citation

Mushroom lectins: specificity, structure and bioactivity relevant to human disease.
Hassan et al. (2015)
5 more