› 4ah3

X-ray diffraction
1.57Å resolution

Crystal structure of the holo omega-transaminase from Chromobacterium violaceum

Released:
DOI: 10.2210/pdb4ah3/pdb
PDB entry 4ah3 coloured by chain, front view.
PDB entry 4ah3 coloured by chain, side view.
PDB entry 4ah3 coloured by chain, top view.
Source organism: Chromobacterium violaceum
Primary publication:
Structural studies of Pseudomonas and Chromobacterium ω-aminotransferases provide insights into their differing substrate specificity.
Sayer C, Isupov MN, Westlake A, Littlechild JA
Acta Crystallogr D Biol Crystallogr 69 564-76 (2013)
PMID: 23519665

Function and Biology Details

Reaction catalysed: 
L-alanine + 3-oxopropanoate = pyruvate + beta-alanine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181841 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative 8-amino-7-oxononanoate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 459 amino acids
Theoretical weight: 51.28 KDa
Source organism: Chromobacterium violaceum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q7NWG4 (Residues: 1-459; Coverage: 100%)
Gene name: CV_2025
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 4 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX10.1
Spacegroup: P1
Unit cell:
a: 61.89Å b: 62.23Å c: 119.61Å
α: 75.14° β: 81.73° γ: 76.17°
R-values:
R R work R free
0.225 0.222 0.273
Expression system: Escherichia coli BL21

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Citations

2 review citations

Recent achievements in developing the biocatalytic toolbox for chiral amine synthesis.
Kohls et al. (2014)
1 more

6 mentions without citation

Crystal structure of an (R)-selective ω-transaminase from Aspergillus terreus.
Łyskowski et al. (2014)
5 more