› 4aha

X-ray diffraction
2.2Å resolution

Crystal Structure of Fucose binding lectin from Aspergillus Fumigatus (AFL) in complex with fucosylated monosaccharides (Fuc1-2Gal, Fuc1- 3GlcNAc, Fuc1-4GlcNAc and Fuc1-6GlcNAc)

Released:
DOI: 10.2210/pdb4aha/pdb
PDB entry 4aha coloured by chain, front view.
PDB entry 4aha coloured by chain, side view.
PDB entry 4aha coloured by chain, top view.
Source organism: Aspergillus fumigatus
Primary publication:
Structural insights into Aspergillus fumigatus lectin specificity: AFL binding sites are functionally non-equivalent.
Houser J, Komarek J, Cioci G, Varrot A, Imberty A, Wimmerova M
Acta Crystallogr D Biol Crystallogr 71 442-53 (2015)
PMID: 25760594

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175847 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Fucose-specific lectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 34.61 KDa
Source organism: Aspergillus fumigatus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q4WW81 (Residues: 1-315; Coverage: 100%)
Gene names: AFUA_5G14740, fleA
Sequence domains: Fungal fucose-specific lectin
Structure domains: Fucose-specific lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: FUC, NAG
Carbohydrate polymer
1 bound ligand:
Ligand FUC 8 x FUC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 45.73Å b: 88.38Å c: 78.58Å
α: 90° β: 99.63° γ: 90°
R-values:
R R work R free
0.167 0.165 0.212
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Histo-blood group antigens as mediators of infections.
Heggelund et al. (2017)
1 more

3 mentions without citation

Gold(i)-catalyzed [2 + 2 + 2] cycloaddition of allenamides, alkenes and aldehydes: a straightforward approach to tetrahydropyrans.
Faustino et al. (2015)
2 more