› 4aij

X-ray diffraction
2.05Å resolution

Crystal structure of RovA from Yersinia in complex with a rovA promoter fragment

Released:
DOI: 10.2210/pdb4aij/pdb
PDB entry 4aij coloured by chain, front view.
PDB entry 4aij coloured by chain, side view.
PDB entry 4aij coloured by chain, top view.
Source organism: Yersinia pseudotuberculosis
Primary publication:
Structural basis for intrinsic thermosensing by the master virulence regulator RovA of Yersinia.
Quade N, Mendonca C, Herbst K, Heroven AK, Ritter C, Heinz DW, Dersch P
J Biol Chem 287 35796-803 (2012)
PMID: 22936808

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109315 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcriptional regulator SlyA Chains: A, B
Molecule details ›
Chains: A, B
Length: 151 amino acids
Theoretical weight: 17.23 KDa
Source organism: Yersinia pseudotuberculosis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: B1JJ73 (Residues: 1-143; Coverage: 100%)
Gene names: YPK_1876, slyA
Sequence domains: MarR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
ROVA PROMOTER FRAGMENT, 5'-D(*AP*AP*TP*TP*AP*TP*AP*TP *TP*AP*TP*TP*TP*GP*AP*AP*TP*TP*AP*AP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.45 KDa
Source organism: Yersinia pseudotuberculosis
Expression system: Not provided
ROVA PROMOTER FRAGMENT, 5'-D(*TP*AP*TP*TP*AP*AP*TP*TP *CP*AP*AP*AP*TP*AP*AP*TP*AP*TP*AP*AP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.43 KDa
Source organism: Yersinia pseudotuberculosis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3
Unit cell:
a: 88.779Å b: 88.779Å c: 67.729Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.203 0.251
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

9 review citations

Bacterial Stress Responses during Host Infection.
Fang et al. (2016)
8 more

3 mentions without citation

Structural basis for intrinsic thermosensing by the master virulence regulator RovA of Yersinia.
Quade et al. (2012)
2 more