› 4aix

X-ray diffraction
1.8Å resolution

Crystallographic structure of an amyloidogenic variant, 3rC34Y, of the germinal line lambda 3

Released:
DOI: 10.2210/pdb4aix/pdb
PDB entry 4aix coloured by chain, front view.
PDB entry 4aix coloured by chain, side view.
PDB entry 4aix coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Site-directed mutagenesis reveals regions implicated in the stability and fiber formation of human λ3r light chains.
Villalba MI, Canul-Tec JC, Luna-Martínez OD, Sánchez-Alcalá R, Olamendi-Portugal T, Rudiño-Piñera E, Rojas S, Sánchez-López R, Fernández-Velasco DA, Becerril B
J Biol Chem 290 2577-92 (2015)
PMID: 25505244

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
IG LAMBDA CHAIN V-IV REGION BAU Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 107 amino acids
Theoretical weight: 11.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P31
Unit cell:
a: 41.884Å b: 41.884Å c: 170.807Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.168 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

Concurrent structural and biophysical traits link with immunoglobulin light chains amyloid propensity.
Oberti et al. (2017)
5 more

3 mentions without citation

Affinity maturation is required for pathogenic monovalent IgG4 autoantibody development in myasthenia gravis.
Fichtner et al. (2020)
2 more