› 4aj0

X-ray diffraction
1.7Å resolution

Crystallographic structure of an amyloidogenic variant, 3rCW, of the germinal line lambda 3

Released:
DOI: 10.2210/pdb4aj0/pdb
PDB entry 4aj0 coloured by chain, front view.
PDB entry 4aj0 coloured by chain, side view.
PDB entry 4aj0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Site-directed mutagenesis reveals regions implicated in the stability and fiber formation of human λ3r light chains.
Villalba MI, Canul-Tec JC, Luna-Martínez OD, Sánchez-Alcalá R, Olamendi-Portugal T, Rudiño-Piñera E, Rojas S, Sánchez-López R, Fernández-Velasco DA, Becerril B
J Biol Chem 290 2577-92 (2015)
PMID: 25505244

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213442 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GERMINAL LINE LAMBDA 3 3RCW VARIANT Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 107 amino acids
Theoretical weight: 11.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand PG0 2 x PG0
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P31
Unit cell:
a: 41.807Å b: 41.807Å c: 171.035Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 citation in other articles

Concurrent structural and biophysical traits link with immunoglobulin light chains amyloid propensity.
Oberti et al. (2017)
6 more

2 mentions without citation

Affinity maturation is required for pathogenic monovalent IgG4 autoantibody development in myasthenia gravis.
Fichtner et al. (2020)
1 more