› 4ak6

X-ray diffraction
1.9Å resolution

BpGH117_H302E mutant glycoside hydrolase

Released:
DOI: 10.2210/pdb4ak6/pdb
PDB entry 4ak6 coloured by chain, front view.
PDB entry 4ak6 coloured by chain, side view.
PDB entry 4ak6 coloured by chain, top view.
Source organism: Phocaeicola plebeius
Primary publication:
Analysis of keystone enzyme in Agar hydrolysis provides insight into the degradation (of a polysaccharide from) red seaweeds.
Hehemann JH, Smyth L, Yadav A, Vocadlo DJ, Boraston AB
J Biol Chem 287 13985-95 (2012)
PMID: 22393053

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110184 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 404 amino acids
Theoretical weight: 45.94 KDa
Source organism: Phocaeicola plebeius
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B5CY74 (Residues: 22-402; Coverage: 99%)
Gene name: BACPLE_01671
Sequence domains: Glycosyl hydrolases family 43
Structure domains: Glycosyl hydrolase domain; family 43

Ligands and Environments

1 bound ligand:
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 85.26Å b: 96.55Å c: 103.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.216 0.252
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Microorganisms living on macroalgae: diversity, interactions, and biotechnological applications.
Martin et al. (2014)
7 more

1 mention without citation

Analysis of keystone enzyme in Agar hydrolysis provides insight into the degradation (of a polysaccharide from) red seaweeds.
Hehemann et al. (2012)