› 4akj

X-ray diffraction
2.01Å resolution

Ligand controlled assembly of hexamers, dihexamers, and linear multihexamer structures by an engineered acylated insulin

Released:
DOI: 10.2210/pdb4akj/pdb
PDB entry 4akj coloured by chain, front view.
PDB entry 4akj coloured by chain, side view.
PDB entry 4akj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ligand-controlled assembly of hexamers, dihexamers, and linear multihexamer structures by the engineered acylated insulin degludec.
Steensgaard DB, Schluckebier G, Strauss HM, Norrman M, Thomsen JK, Friderichsen AV, Havelund S, Jonassen I
Biochemistry 52 295-309 (2013)
PMID: 23256685

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134224 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 29 amino acids
Theoretical weight: 3.33 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 25-53; Coverage: 34%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

4 bound ligands:
Ligand IPH 2 x IPH
Ligand 16E 1 x 16E
Ligand ZN 2 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: R3
Unit cell:
a: 78.717Å b: 78.717Å c: 40.551Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.196 0.229
Expression system: Saccharomyces cerevisiae

Quick links

Citations

17 review citations

A review of the pharmacological properties of insulin degludec and their clinical relevance.
Haahr et al. (2014)
16 more

2 mentions without citation

How Structural Biologists and the Protein Data Bank Contributed to Recent FDA New Drug Approvals.
Westbrook et al. (2019)
1 more