› 4akr

X-ray diffraction
2.2Å resolution

Crystal Structure of the cytoplasmic actin capping protein Cap32_34 from Dictyostelium discoideum

Released:
DOI: 10.2210/pdb4akr/pdb
PDB entry 4akr coloured by chain, front view.
PDB entry 4akr coloured by chain, side view.
PDB entry 4akr coloured by chain, top view.
Source organism: Dictyostelium discoideum AX2
Primary publication:
Conservation and divergence between cytoplasmic and muscle-specific actin capping proteins: insights from the crystal structure of cytoplasmic Cap32/34 from Dictyostelium discoideum.
Eckert C, Goretzki A, Faberova M, Kollmar M
BMC Struct Biol 12 12 (2012)
PMID: 22657106

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146439 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
F-actin-capping protein subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 281 amino acids
Theoretical weight: 31.19 KDa
Source organism: Dictyostelium discoideum AX2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13022 (Residues: 1-281; Coverage: 100%)
Gene names: DDB_G0272104, abpD, acpB
Sequence domains: F-actin capping protein alpha subunit
Structure domains:
F-actin-capping protein subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 290 amino acids
Theoretical weight: 32.87 KDa
Source organism: Dictyostelium discoideum AX2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13021 (Residues: 2-272; Coverage: 100%)
Gene names: DDB_G0267374, abpE, acpA
Sequence domains: F-actin capping protein, beta subunit
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P41
Unit cell:
a: 124.46Å b: 124.46Å c: 77.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.265
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

Structural and kinetic bases for the metal preference of the M18 aminopeptidase from Pseudomonas aeruginosa.
Nguyen et al. (2014)
2 more

1 mention without citation

Conservation and divergence between cytoplasmic and muscle-specific actin capping proteins: insights from the crystal structure of cytoplasmic Cap32/34 from Dictyostelium discoideum.
Eckert et al. (2012)