› 4akz

X-ray diffraction
2.25Å resolution

CRYSTAL STRUCTURE OF VIRB8 FROM BRUCELLA SUIS

Released:
DOI: 10.2210/pdb4akz/pdb
PDB entry 4akz coloured by chain, front view.
PDB entry 4akz coloured by chain, side view.
PDB entry 4akz coloured by chain, top view.
Source organism: Brucella suis
Primary publication:
Identification of the binding site of Brucella VirB8 interaction inhibitors.
Smith MA, Coinçon M, Paschos A, Jolicoeur B, Lavallée P, Sygusch J, Baron C
Chem Biol 19 1041-8 (2012)
PMID: 22921071

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type IV secretion system protein virB8 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 138 amino acids
Theoretical weight: 15.54 KDa
Source organism: Brucella suis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7CEG3 (Residues: 97-234; Coverage: 58%)
Gene names: BRA0062, BS1330_II0062, virB8
Sequence domains: VirB8 protein
Structure domains: VirB8 protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I4122
Unit cell:
a: 198.44Å b: 198.44Å c: 103.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

The effects of antibiotics on the microbiome throughout development and alternative approaches for therapeutic modulation.
Langdon et al. (2016)
20 more

3 mentions without citation

Structural Analysis and Inhibition of TraE from the pKM101 Type IV Secretion System.
Casu et al. (2016)
2 more