4al6

X-ray diffraction
2.63Å resolution

Crystal structure of the S148ACsy4-crRNA complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-169803 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
CRISPR-associated endonuclease Cas6/Csy4 Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 21.8 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM2 (Residues: 1-187; Coverage: 100%)
Gene names: PA14_33300, cas6f, csy4
Sequence domains: CRISPR-associated protein (Cas_Csy4)
Structure domains: CRISPR-associated endoribonuclease Cas6/Csy4
CRRNA, 5'-R(CP*UP*GP*CP*CP*GP*UP*AP*UP*AP*GP*GP* CP*AP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 5.11 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2
Unit cell:
a: 62.39Å b: 46.88Å c: 87.39Å
α: 90° β: 107.24° γ: 90°
R-values:
R R work R free
0.203 0.2 0.252
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided