› 4am7

X-ray diffraction
3.25Å resolution

ADP-BOUND C-TERMINAL DOMAIN OF ACTIN-RELATED PROTEIN ARP8 FROM S. CEREVISIAE

Released:
DOI: 10.2210/pdb4am7/pdb
PDB entry 4am7 coloured by chain, front view.
PDB entry 4am7 coloured by chain, side view.
PDB entry 4am7 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Interactions between the nucleosome histone core and Arp8 in the INO80 chromatin remodeling complex.
Saravanan M, Wuerges J, Bose D, McCormack EA, Cook NJ, Zhang X, Wigley DB
Proc Natl Acad Sci U S A 109 20883-8 (2012)
PMID: 23213201

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171348 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin-like protein ARP8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 655 amino acids
Theoretical weight: 74.81 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli B
UniProt:
  • Canonical: Q12386 (Residues: 248-881; Coverage: 72%)
Gene names: ARP8, YOR141C, YOR3348C
Sequence domains: Actin
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 137.718Å b: 89.795Å c: 149.647Å
α: 90° β: 114.37° γ: 90°
R-values:
R R work R free
0.223 0.219 0.284
Expression system: Escherichia coli B

Quick links

Citations

8 review citations

Nucleosome structure and function.
McGinty et al. (2015)
7 more

1 mention without citation

Interactions between the nucleosome histone core and Arp8 in the INO80 chromatin remodeling complex.
Saravanan et al. (2012)