› 4am8

X-ray diffraction
1.99Å resolution

Crystal structure of the R54G mutant of putrescine transcarbamylase from Enterococcus faecalis bound to a curing guanidinium ion

Released:
DOI: 10.2210/pdb4am8/pdb
PDB entry 4am8 coloured by chain, front view.
PDB entry 4am8 coloured by chain, side view.
PDB entry 4am8 coloured by chain, top view.
Source organism: Enterococcus faecalis
Entry authors: Polo LM, Rubio V

Function and Biology Details

Reaction catalysed: 
Carbamoyl phosphate + putrescine = phosphate + N-carbamoylputrescine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182851 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putrescine carbamoyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 355 amino acids
Theoretical weight: 40.04 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q837U7 (Residues: 1-339; Coverage: 100%)
Gene names: EF_0732, agcB, argF-2, ptcA
Sequence domains:
Structure domains: Aspartate/ornithine carbamoyltransferase

Ligands and Environments

6 bound ligands:
Ligand GLN 3 x GLN
Ligand PAO 6 x PAO
Ligand GAI 7 x GAI
Ligand TRS 4 x TRS
Ligand NI 2 x NI
Ligand ILE 1 x ILE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Unit cell:
a: 103.69Å b: 130.6Å c: 164.157Å
α: 90° β: 104.25° γ: 90°
R-values:
R R work R free
0.183 0.182 0.206
Expression system: Escherichia coli BL21(DE3)

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