› 4amb

X-ray diffraction
2.62Å resolution

Crystal structure of the glycosyltransferase SnogD from Streptomyces nogalater

Released:
DOI: 10.2210/pdb4amb/pdb
PDB entry 4amb coloured by chain, front view.
PDB entry 4amb coloured by chain, side view.
PDB entry 4amb coloured by chain, top view.
Source organism: Streptomyces nogalater
Primary publication:
Crystal structure of the glycosyltransferase SnogD from the biosynthetic pathway of nogalamycin in Streptomyces nogalater.
Claesson M, Siitonen V, Dobritzsch D, Metsä-Ketelä M, Schneider G
FEBS J 279 3251-63 (2012)
PMID: 22804797

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193211 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative glycosyl transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 400 amino acids
Theoretical weight: 41.95 KDa
Source organism: Streptomyces nogalater
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RN61 (Residues: 13-390; Coverage: 97%)
Gene name: snogD
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
Ligand DUD 1 x DUD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 64.911Å b: 170.955Å c: 69.427Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.223 0.297
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Glycosyltransferases: mechanisms and applications in natural product development.
Liang et al. (2015)
1 more

1 mention without citation

Bifunctional Opioid/Melanocortin Peptidomimetics for Use in Neuropathic Pain: Variation in the Type and Length of the Linker Connecting the Two Pharmacophores.
Witkowska et al. (2022)