› 4amv

X-ray diffraction
2.05Å resolution

E.COLI GLUCOSAMINE-6P SYNTHASE IN COMPLEX WITH FRUCTOSE-6P

Released:
DOI: 10.2210/pdb4amv/pdb
PDB entry 4amv coloured by chain, front view.
PDB entry 4amv coloured by chain, side view.
PDB entry 4amv coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Glutamine binding opens the ammonia channel and activates glucosamine-6P synthase.
Mouilleron S, Badet-Denisot MA, Golinelli-Pimpaneau B
J Biol Chem 281 4404-12 (2006)
PMID: 16339762

Function and Biology Details

Reaction catalysed: 
L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147884 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 609 amino acids
Theoretical weight: 66.95 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli HB101
UniProt:
  • Canonical: P17169 (Residues: 1-609; Coverage: 100%)
Gene names: JW3707, b3729, glmS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand F6R 3 x F6R
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 132.197Å b: 109.677Å c: 176.332Å
α: 90° β: 97.11° γ: 90°
R-values:
R R work R free
0.208 0.206 0.24
Expression system: Escherichia coli HB101

Quick links

Citations

9 review citations

Gates of enzymes.
Gora et al. (2013)
8 more

6 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
5 more