› 4ang

X-ray diffraction
3.5Å resolution

Small RNA phage PRR1 in complex with an RNA operator fragment

Released:
DOI: 10.2210/pdb4ang/pdb
PDB entry 4ang coloured by chain, front view.
PDB entry 4ang coloured by chain, side view.
PDB entry 4ang coloured by chain, top view.
Source organism: Pseudomonas phage PRR1
Primary publication:
PRR1 coat protein binding to its RNA translational operator.
Persson M, Tars K, Liljas L
Acta Crystallogr D Biol Crystallogr 69 367-72 (2013)
PMID: 23519411

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 300-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137178 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Capsid protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 131 amino acids
Theoretical weight: 14.57 KDa
Source organism: Pseudomonas phage PRR1
Expression system: Escherichia coli
UniProt:
  • Canonical: P03616 (Residues: 1-131; Coverage: 100%)
Sequence domains: Levivirus coat protein
Structure domains: MS2 Viral Coat Protein
5'-R(*CP*CP*AP*UP*AP*AP*GP*GP*AP*GP*CP*UP*AP*CP *CP*UP*AP*UP*GP*GP)-3' Chains: R, S
Molecule details ›
Chains: R, S
Length: 20 nucleotides
Theoretical weight: 6.41 KDa
Source organism: Pseudomonas phage PRR1
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 281.3Å b: 285.4Å c: 472.9Å
α: 93.23° β: 90.03° γ: 119.45°
R-values:
R R work R free
0.308 0.308 0.313
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Multilevel Regulation and Translational Switches in Synthetic Biology.
Kopniczky et al. (2015)
2 more