› 4anj

X-ray diffraction
2.6Å resolution

MYOSIN VI (MDinsert2-GFP fusion) PRE-POWERSTROKE STATE (MG.ADP.AlF4)

Released:
DOI: 10.2210/pdb4anj/pdb
PDB entry 4anj coloured by chain, front view.
PDB entry 4anj coloured by chain, side view.
PDB entry 4anj coloured by chain, top view.
Source organisms:
Primary publication:
Processive steps in the reverse direction require uncoupling of the lead head lever arm of myosin VI.
Ménétrey J, Isabet T, Ropars V, Mukherjea M, Pylypenko O, Liu X, Perez J, Vachette P, Sweeney HL, Houdusse AM
Mol Cell 48 75-86 (2012)
PMID: 22940248

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154515 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Green fluorescent protein; Unconventional myosin-VI Chain: A
Molecule details ›
Chain: A
Length: 1052 amino acids
Theoretical weight: 120.13 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q29122 (Residues: 1-818; Coverage: 65%)
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene names: GFP, MYO6
Sequence domains:
Structure domains:
Calmodulin Chain: B
Molecule details ›
Chain: B
Length: 149 amino acids
Theoretical weight: 16.83 KDa
Source organism: Drosophila melanogaster
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62152 (Residues: 1-149; Coverage: 100%)
Gene names: CG8472, Cam
Sequence domains: EF-hand domain pair

Ligands and Environments

4 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
Ligand ALF 1 x ALF
Ligand CA 3 x CA
1 modified residue:
Ligand CR2 1 x CR2

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 193.093Å b: 62.657Å c: 156.04Å
α: 90° β: 117.96° γ: 90°
R-values:
R R work R free
0.241 0.239 0.288
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Force generation by kinesin and myosin cytoskeletal motor proteins.
Kull et al. (2013)
1 more

7 mentions without citation

Catalytic strategy used by the myosin motor to hydrolyze ATP.
Kiani et al. (2014)
6 more