› 4ap9

X-ray diffraction
1.78Å resolution

Crystal structure of phosphoserine phosphatase from T. onnurineus in complex with NDSB-201

Released:
DOI: 10.2210/pdb4ap9/pdb
PDB entry 4ap9 coloured by chain, front view.
PDB entry 4ap9 coloured by chain, side view.
PDB entry 4ap9 coloured by chain, top view.
Source organism: Thermococcus onnurineus
Primary publication:
Identification of a novel ligand binding site in phosphoserine phosphatase from the hyperthermophilic archaeon Thermococcus onnurineus.
Jung TY, Kim YS, Oh BH, Woo E
Proteins 81 819-29 (2013)
PMID: 23239422

Function and Biology Details

Reaction catalysed: 
O-phospho-L(or D)-serine + H(2)O = L(or D)-serine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
phosphoserine phosphatase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 201 amino acids
Theoretical weight: 23.35 KDa
Source organism: Thermococcus onnurineus
Expression system: Escherichia coli
UniProt:
  • Canonical: B6YX36 (Residues: 1-194; Coverage: 100%)
Gene name: TON_1161
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 1PS 6 x 1PS
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P4
Unit cell:
a: 128.131Å b: 128.131Å c: 63.077Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.195 0.221
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

The path to triacylglyceride obesity in the sta6 strain of Chlamydomonas reinhardtii.
Goodenough et al. (2014)
3 more