› 4aqx

X-ray diffraction
2.2Å resolution

Crystal structure of I-CreI complexed with its target methylated at position plus 2 (in the b strand) in the presence of magnesium

Released:
DOI: 10.2210/pdb4aqx/pdb
PDB entry 4aqx coloured by chain, front view.
PDB entry 4aqx coloured by chain, side view.
PDB entry 4aqx coloured by chain, top view.
Source organisms:
Primary publication:
5'-Cytosine-phosphoguanine (CpG) methylation impacts the activity of natural and engineered meganucleases.
Valton J, Daboussi F, Leduc S, Molina R, Redondo P, Macmaster R, Montoya G, Duchateau P
J Biol Chem 287 30139-50 (2012)
PMID: 22740697

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117218 (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
DNA endonuclease I-CreI Chains: A, B
Molecule details ›
Chains: A, B
Length: 152 amino acids
Theoretical weight: 17.52 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 2-153; Coverage: 93%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP)-3' Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.29 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*GP*AP*CP*AP*GP*TP*TP*TP*GP*GP)-3' Chain: D
Molecule details ›
Chain: D
Length: 10 nucleotides
Theoretical weight: 3.1 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*CP*CP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*5CM)-3' Chain: E
Molecule details ›
Chain: E
Length: 14 nucleotides
Theoretical weight: 4.2 KDa
Source organism: synthetic construct
Expression system: Not provided
5'-D(*GP*AP*CP*GP*TP*TP*TP*TP*GP*AP)-3' Chain: F
Molecule details ›
Chain: F
Length: 10 nucleotides
Theoretical weight: 3.08 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand GOL 5 x GOL
Ligand MG 3 x MG
1 modified residue:
Ligand 5CM 1 x 5CM

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Unit cell:
a: 45.839Å b: 71.401Å c: 179.054Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.242
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Plant genome engineering with sequence-specific nucleases.
Voytas DF. (2013)
3 more

1 mention without citation

5'-Cytosine-phosphoguanine (CpG) methylation impacts the activity of natural and engineered meganucleases.
Valton et al. (2012)