› 4asc

X-ray diffraction
1.78Å resolution

Crystal structure of the Kelch domain of human KBTBD5

Released:
DOI: 10.2210/pdb4asc/pdb
PDB entry 4asc coloured by chain, front view.
PDB entry 4asc coloured by chain, side view.
PDB entry 4asc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for Cul3 protein assembly with the BTB-Kelch family of E3 ubiquitin ligases.
Canning P, Cooper CDO, Krojer T, Murray JW, Pike ACW, Chaikuad A, Keates T, Thangaratnarajah C, Hojzan V, Marsden BD, Gileadi O, Knapp S, von Delft F, Bullock AN
J Biol Chem 288 7803-7814 (2013)
PMID: 23349464

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kelch-like protein 40 Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 35.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2TBA0 (Residues: 314-621; Coverage: 50%)
Gene names: KBTBD5, KLHL40, SRYP
Sequence domains: Kelch motif
Structure domains: Kelch-type beta propeller

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 61.33Å b: 64.95Å c: 89.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

44 review citations

The KEAP1-NRF2 System: a Thiol-Based Sensor-Effector Apparatus for Maintaining Redox Homeostasis.
Yamamoto et al. (2018)
43 more

6 mentions without citation

Challenges and Limitations of Targeting the Keap1-Nrf2 Pathway for Neurotherapeutics: Bach1 De-Repression to the Rescue.
Hushpulian et al. (2021)
5 more