› 4aup

X-ray diffraction
1.9Å resolution

Tuber borchii Phospholipase A2

Released:
DOI: 10.2210/pdb4aup/pdb
PDB entry 4aup coloured by chain, front view.
PDB entry 4aup coloured by chain, side view.
PDB entry 4aup coloured by chain, top view.
Source organism: Tuber borchii
Primary publication:
Autoproteolytic Activation of a Symbiosis-regulated Truffle Phospholipase A2.
Cavazzini D, Meschi F, Corsini R, Bolchi A, Rossi GL, Einsle O, Ottonello S
J Biol Chem 288 1533-47 (2013)
PMID: 23192346

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192852 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2 group XIII Chains: A, B
Molecule details ›
Chains: A, B
Length: 123 amino acids
Theoretical weight: 14.21 KDa
Source organism: Tuber borchii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9P4F6 (Residues: 89-211; Coverage: 64%)
Sequence domains: Prokaryotic phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

2 bound ligands:
Ligand ACT 2 x ACT
Ligand SCN 1 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P212121
Unit cell:
a: 34.498Å b: 66.837Å c: 117.384Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.21 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 citation in other articles

Posttranslational Protein Modifications in Plant Metabolism.
Friso et al. (2015)
7 more

1 mention without citation

Autoproteolytic Activation of a Symbiosis-regulated Truffle Phospholipase A2.
Cavazzini et al. (2013)