› 4av3

X-ray diffraction
2.6Å resolution

Crystal structure of Thermotoga Maritima sodium pumping membrane integral pyrophosphatase with metal ions in active site

Released:
DOI: 10.2210/pdb4av3/pdb
PDB entry 4av3 coloured by chain, front view.
PDB entry 4av3 coloured by chain, side view.
PDB entry 4av3 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
The structure and catalytic cycle of a sodium-pumping pyrophosphatase.
Kellosalo J, Kajander T, Kogan K, Pokharel K, Goldman A
Science 337 473-6 (2012)
PMID: 22837527

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
K(+)-stimulated pyrophosphate-energized sodium pump Chains: A, B
Molecule details ›
Chains: A, B
Length: 735 amino acids
Theoretical weight: 78.22 KDa
Source organism: Thermotoga maritima
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q9S5X0 (Residues: 2-726; Coverage: 100%)
Gene names: TM_0174, hppA
Sequence domains: Inorganic H+ pyrophosphatase

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21
Unit cell:
a: 83.52Å b: 107.779Å c: 102.521Å
α: 90° β: 108.5° γ: 90°
R-values:
R R work R free
0.207 0.205 0.245
Expression system: Saccharomyces cerevisiae

Quick links

Citations

9 review citations

Membrane protein structure determination - the next generation.
Moraes et al. (2014)
8 more

11 mentions without citation

The Function of Membrane Integral Pyrophosphatases From Whole Organism to Single Molecule.
Holmes et al. (2019)
10 more