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› 4av6

X-ray diffraction
4Å resolution

Crystal structure of Thermotoga maritima sodium pumping membrane integral pyrophosphatase at 4 A in complex with phosphate and magnesium

Released:
DOI: 10.2210/pdb4av6/pdb
Model geometry
Fit model/data
PDB entry 4av6 coloured by chain, front view.
PDB entry 4av6 coloured by chain, side view.
PDB entry 4av6 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
The structure and catalytic cycle of a sodium-pumping pyrophosphatase.
Kellosalo J, Kajander T, Kogan K, Pokharel K, Goldman A
Science 337 473-6 (2012)
PMID: 22837527

Function and Biology Details

Reaction catalysed: 
Na(+)(in) + diphosphate + H2O = Na(+)(out) + 2 phosphate + H(+).
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193409 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
K(+)-stimulated pyrophosphate-energized sodium pump Chains: A, B
Molecule details ›
Chains: A, B
Length: 735 amino acids
Theoretical weight: 78.22 KDa
Source organism: Thermotoga maritima
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q9S5X0 (Residues: 2-726; Coverage: 100%)
Gene names: TM_0174, hppA
Sequence domains: Inorganic H+ pyrophosphatase

Ligands and Environments

3 bound ligands:
Ligand PO4 4 x PO4
Ligand MG 8 x MG
Ligand K 2 x K
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 85.8Å b: 112.266Å c: 109.714Å
α: 90° β: 103° γ: 90°
R-values:
R R work R free
0.299 0.295 0.365
Expression system: Saccharomyces cerevisiae

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Citations

9 review citations

Membrane protein structure determination - the next generation.
Moraes et al. (2014)
8 more

6 mentions without citation

The Function of Membrane Integral Pyrophosphatases From Whole Organism to Single Molecule.
Holmes et al. (2019)
5 more