› 4aw4

X-ray diffraction
1.93Å resolution

Engineered variant of Listeria monocytogenes InlB internalin domain with an additional leucine rich repeat inserted

Released:
DOI: 10.2210/pdb4aw4/pdb
PDB entry 4aw4 coloured by chain, front view.
PDB entry 4aw4 coloured by chain, side view.
PDB entry 4aw4 coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Primary publication:
Engineered variants of InlB with an additional leucine-rich repeat discriminate between physiologically relevant and packing contacts in crystal structures of the InlB:MET complex.
Niemann HH, Gherardi E, Bleymüller WM, Heinz DW
Protein Sci 21 1528-39 (2012)
PMID: 22887347

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144112 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Internalin B Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 311 amino acids
Theoretical weight: 34.68 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DQD3 (Residues: 36-98, 99-321; Coverage: 48%)
Gene name: inlB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 22 x SO4
Ligand GOL 11 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: C2
Unit cell:
a: 179.08Å b: 102.15Å c: 69.81Å
α: 90° β: 90.23° γ: 90°
R-values:
R R work R free
0.168 0.167 0.205
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Structural basis of MET receptor dimerization by the bacterial invasion protein InlB and the HGF/SF splice variant NK1.
Niemann HH. (2013)
4 other articles

1 mention without citation

Structure-Guided Exploration of SDS22 Interactions with Protein Phosphatase PP1 and the Splicing Factor BCLAF1.
Heroes et al. (2019)