› 4awg

X-ray diffraction
2.6Å resolution

Influenza strain pH1N1 2009 polymerase subunit PA endonuclease in complex with diketo compound 3

Released:
DOI: 10.2210/pdb4awg/pdb
PDB entry 4awg coloured by chain, front view.
PDB entry 4awg coloured by chain, side view.
PDB entry 4awg coloured by chain, top view.
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Primary publication:
Structural analysis of specific metal chelating inhibitor binding to the endonuclease domain of influenza pH1N1 (2009) polymerase.
Kowalinski E, Zubieta C, Wolkerstorfer A, Szolar OH, Ruigrok RW, Cusack S
PLoS Pathog 8 e1002831 (2012)
PMID: 22876177

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111233 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase acidic protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 204 amino acids
Theoretical weight: 23.94 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3W5S0 (Residues: 1-198; Coverage: 28%)
Gene name: PA
Structure domains: Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain

Ligands and Environments

3 bound ligands:
Ligand SO4 14 x SO4
Ligand CI3 4 x CI3
Ligand MN 8 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 54.57Å b: 122.54Å c: 129.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.19 0.262
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

Influenza virus RNA polymerase: insights into the mechanisms of viral RNA synthesis.
Te Velthuis et al. (2016)
24 more

1 mention without citation

Structural analysis of specific metal chelating inhibitor binding to the endonuclease domain of influenza pH1N1 (2009) polymerase.
Kowalinski et al. (2012)