› 4awn

X-ray diffraction
1.95Å resolution

Structure of recombinant human DNase I (rhDNaseI) in complex with Magnesium and Phosphate.

Released:
DOI: 10.2210/pdb4awn/pdb
PDB entry 4awn coloured by chain, front view.
PDB entry 4awn coloured by chain, side view.
PDB entry 4awn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of human DNase I bound to magnesium and phosphate ions points to a catalytic mechanism common to members of the DNase I-like superfamily.
Parsiegla G, Noguere C, Santell L, Lazarus RA, Bourne Y
Biochemistry 51 10250-8 (2012)
PMID: 23215638

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Deoxyribonuclease-1 Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.28 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P24855 (Residues: 23-282; Coverage: 100%)
Gene names: DNASE1, DNL1, DRNI
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
5 bound ligands:
Ligand CA 2 x CA
Ligand MG 1 x MG
Ligand PO4 1 x PO4
Ligand BTB 1 x BTB
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 41.96Å b: 58.66Å c: 96.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.166 0.218
Expression system: Cricetulus griseus

Quick links

Citations

2 review citations

Biologically inspired strategies for combating bacterial biofilms.
Blackledge et al. (2013)
1 more

7 mentions without citation

Association of the DNASE1L3 rs35677470 polymorphism with systemic lupus erythematosus, rheumatoid arthritis and systemic sclerosis: Structural biological insights.
Zervou et al. (2020)
6 more