› 4ax3

X-ray diffraction
1.6Å resolution

Structure of three-domain heme-Cu nitrite reductase from Ralstonia pickettii at 1.6 A resolution

Released:
DOI: 10.2210/pdb4ax3/pdb
PDB entry 4ax3 coloured by chain, front view.
PDB entry 4ax3 coloured by chain, side view.
PDB entry 4ax3 coloured by chain, top view.
Source organism: Ralstonia pickettii 12J
Primary publication:
Structures of protein-protein complexes involved in electron transfer.
Antonyuk SV, Han C, Eady RR, Hasnain SS
Nature 496 123-6 (2013)
PMID: 23535590

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109731 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 468 amino acids
Theoretical weight: 49.95 KDa
Source organism: Ralstonia pickettii 12J
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B2UHR8 (Residues: 32-499; Coverage: 100%)
Gene name: Rpic_4015
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEC 4 x HEC
1 bound ligand:
Ligand CU 8 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P213
Unit cell:
a: 185.812Å b: 185.812Å c: 185.812Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.163 0.194
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Recent structural insights into the function of copper nitrite reductases.
Horrell et al. (2017)
3 more

4 mentions without citation

Structures of protein-protein complexes involved in electron transfer.
Antonyuk et al. (2013)
3 more