› 4aym

X-ray diffraction
3Å resolution

Structure of a complex between CCPs 6 and 7 of Human Complement Factor H and Neisseria meningitidis FHbp Variant 3 P106A mutant

Released:
DOI: 10.2210/pdb4aym/pdb
PDB entry 4aym coloured by chain, front view.
PDB entry 4aym coloured by chain, side view.
PDB entry 4aym coloured by chain, top view.
Source organisms:
Primary publication:
Design and evaluation of meningococcal vaccines through structure-based modification of host and pathogen molecules.
Johnson S, Tan L, van der Veen S, Caesar J, Goicoechea De Jorge E, Harding RJ, Bai X, Exley RM, Ward PN, Ruivo N, Trivedi K, Cumber E, Jones R, Newham L, Staunton D, Ufret-Vincenty R, Borrow R, Pickering MC, Lea SM, Tang CM
PLoS Pathog 8 e1002981 (2012)
PMID: 23133374

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-140216 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Complement factor H Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 125 amino acids
Theoretical weight: 14.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli B
UniProt:
  • Canonical: P08603 (Residues: 321-443; Coverage: 10%)
Gene names: CFH, HF, HF1, HF2
Sequence domains: Sushi repeat (SCR repeat)
Structure domains: Complement Module, domain 1
Lipoprotein GNA1870 Chains: C, D
Molecule details ›
Chains: C, D
Length: 270 amino acids
Theoretical weight: 29.49 KDa
Source organism: Neisseria meningitidis MC58
Expression system: Escherichia coli B
UniProt:
  • Canonical: Q19KF7 (Residues: 32-281; Coverage: 95%)
Gene name: gna1870
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2221
Unit cell:
a: 78.09Å b: 83.49Å c: 361.43Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.254 0.257
Expression system: Escherichia coli B

Quick links

Citations

12 review citations

Structural vaccinology starts to deliver.
Dormitzer et al. (2012)
11 more

2 mentions without citation

Competition between antagonistic complement factors for a single protein on N. meningitidis rules disease susceptibility.
Caesar et al. (2014)
1 more