› 4b1x

X-ray diffraction
1.8Å resolution

Structure of the Phactr1 RPEL-2 bound to G-actin

Released:
DOI: 10.2210/pdb4b1x/pdb
PDB entry 4b1x coloured by chain, front view.
PDB entry 4b1x coloured by chain, side view.
PDB entry 4b1x coloured by chain, top view.
Source organisms:
Primary publication:
Structures of the Phactr1 RPEL domain and RPEL motif complexes with G-actin reveal the molecular basis for actin binding cooperativity.
Mouilleron S, Wiezlak M, O'Reilly N, Treisman R, McDonald NQ
Structure 20 1960-70 (2012)
PMID: 23041370

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159304 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chain: B
Molecule details ›
Chain: B
Length: 376 amino acids
Theoretical weight: 41.97 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 2-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Phosphatase and actin regulator 1 Chain: M
Molecule details ›
Chain: M
Length: 32 amino acids
Theoretical weight: 3.87 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q2M3X8 (Residues: 455-486; Coverage: 6%)
Gene name: Phactr1
Sequence domains: RPEL repeat

Ligands and Environments

3 bound ligands:
Ligand LAB 1 x LAB
Ligand ATP 1 x ATP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND
Spacegroup: P3221
Unit cell:
a: 77.28Å b: 77.28Å c: 130.809Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.169 0.213
Expression system: Not provided

Quick links

Citations

4 review citations

Guardians of the actin monomer.
Xue et al. (2013)
3 more