› 4b1z

X-ray diffraction
3.3Å resolution

Structure of the Phactr1 RPEL domain bound to G-actin

Released:
DOI: 10.2210/pdb4b1z/pdb
PDB entry 4b1z coloured by chain, front view.
PDB entry 4b1z coloured by chain, side view.
PDB entry 4b1z coloured by chain, top view.
Source organisms:
Primary publication:
Structures of the Phactr1 RPEL domain and RPEL motif complexes with G-actin reveal the molecular basis for actin binding cooperativity.
Mouilleron S, Wiezlak M, O'Reilly N, Treisman R, McDonald NQ
Structure 20 1960-70 (2012)
PMID: 23041370

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159338 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 376 amino acids
Theoretical weight: 41.97 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 2-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Phosphatase and actin regulator 1 Chains: M, N
Molecule details ›
Chains: M, N
Length: 115 amino acids
Theoretical weight: 14.02 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q2M3X8 (Residues: 414-528; Coverage: 20%)
Gene name: Phactr1
Sequence domains: RPEL repeat
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ATP 6 x ATP
Ligand MG 6 x MG
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND
Spacegroup: P212121
Unit cell:
a: 127.06Å b: 142.89Å c: 184.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.213 0.236
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Guardians of the actin monomer.
Xue et al. (2013)
3 more

2 mentions without citation

D-Loop Mutation G42A/G46A Decreases Actin Dynamics.
Matsuzaki et al. (2020)
1 more