› 4b2j

X-ray diffraction
1.9Å resolution

COMPLEXES OF DODECIN WITH FLAVIN AND FLAVIN-LIKE LIGANDS

Released:
DOI: 10.2210/pdb4b2j/pdb
Model geometry
Fit model/data
PDB entry 4b2j coloured by chain, front view.
PDB entry 4b2j coloured by chain, side view.
PDB entry 4b2j coloured by chain, top view.
Source organism: Halobacterium salinarum R1
Primary publication:
Directed manipulation of a flavoprotein photocycle.
Staudt H, Hoesl MG, Dreuw A, Serdjukow S, Oesterhelt D, Budisa N, Wachtveitl J, Grininger M
Angew Chem Int Ed Engl 52 8463-6 (2013)
PMID: 23818044

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109191 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dodecin Chain: A
Molecule details ›
Chain: A
Length: 76 amino acids
Theoretical weight: 8.53 KDa
Source organism: Halobacterium salinarum R1
Expression system: Escherichia coli
UniProt:
  • Canonical: B0R5M0 (Residues: 1-68; Coverage: 100%)
Gene name: OE_3073R
Sequence domains: Dodecin
Structure domains: Flavin-binding protein dodecin

Ligands and Environments

5 bound ligands:
Ligand MG 2 x MG
Ligand NA 1 x NA
Ligand CL 1 x CL
Ligand SO4 1 x SO4
Ligand RBF 1 x RBF
1 modified residue:
Ligand 4FW 1 x 4FW

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: F4132
Unit cell:
a: 142.9Å b: 142.9Å c: 142.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.165 0.186
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Spectroscopic evidence for direct flavin-flavin contact in a bifurcating electron transfer flavoprotein.
Duan et al. (2020)

1 mention without citation

Three-dimensional electron crystallography of protein microcrystals.
Shi et al. (2013)