› 4b3l

X-ray diffraction
2.51Å resolution

Family 1 6-phospho-beta-D glycosidase from Streptococcus pyogenes

Released:
DOI: 10.2210/pdb4b3l/pdb
PDB entry 4b3l coloured by chain, front view.
PDB entry 4b3l coloured by chain, side view.
PDB entry 4b3l coloured by chain, top view.
Source organism: Streptococcus pyogenes
Primary publication:
Structure and activity of the Streptococcus pyogenes family GH1 6-phospho-β-glucosidase SPy1599.
Stepper J, Dabin J, Eklof JM, Thongpoo P, Kongsaeree P, Taylor EJ, Turkenburg JP, Brumer H, Davies GJ
Acta Crystallogr D Biol Crystallogr 69 16-23 (2013)
PMID: 23275159

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 479 amino acids
Theoretical weight: 55.04 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q99YP9 (Residues: 2-480; Coverage: 100%)
Gene name: SPy_1599
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 107.83Å b: 198.04Å c: 107.88Å
α: 90° β: 118.51° γ: 90°
R-values:
R R work R free
0.208 0.205 0.261
Expression system: Escherichia coli BL21

Quick links

Citations

7 citation in other articles

Phylogenomically guided identification of industrially relevant GH1 β-glucosidases through DNA synthesis and nanostructure-initiator mass spectrometry.
Heins et al. (2014)
6 more