› 4b45

X-ray diffraction
2.1Å resolution

CetZ2 from Haloferax volcanii - GTPgS bound protofilament

Released:
DOI: 10.2210/pdb4b45/pdb
PDB entry 4b45 coloured by chain, front view.
PDB entry 4b45 coloured by chain, side view.
PDB entry 4b45 coloured by chain, top view.
Source organism: Haloferax volcanii
Primary publication:
CetZ tubulin-like proteins control archaeal cell shape.
Duggin IG, Aylett CH, Walsh JC, Michie KA, Wang Q, Turnbull L, Dawson EM, Harry EJ, Whitchurch CB, Amos LA, Löwe J
Nature 519 362-5 (2015)
PMID: 25533961

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112621 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tubulin-like protein CetZ2 Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 35.8 KDa
Source organism: Haloferax volcanii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D4GTC1 (Residues: 1-349; Coverage: 97%)
Gene names: C498_15008, HVO_0745, cetZ2, ftsZ3
Sequence domains:
Structure domains:
Tubulin-like protein CetZ2 Chain: B
Molecule details ›
Chain: B
Length: 19 amino acids
Theoretical weight: 2.33 KDa
Source organism: Haloferax volcanii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D4GTC1 (Residues: 350-360; Coverage: 3%)
Gene names: C498_15008, HVO_0745, cetZ2, ftsZ3

Ligands and Environments

1 bound ligand:
Ligand GSP 1 x GSP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 42.427Å b: 43.224Å c: 48.45Å
α: 68.87° β: 75.34° γ: 86.9°
R-values:
R R work R free
0.18 0.177 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Staying in Shape: the Impact of Cell Shape on Bacterial Survival in Diverse Environments.
Yang et al. (2016)
10 more

5 mentions without citation

CetZ tubulin-like proteins control archaeal cell shape.
Duggin et al. (2015)
4 more