› 4b4a

X-ray diffraction
3.5Å resolution

Structure of the TatC core of the twin arginine protein translocation system

Released:
DOI: 10.2210/pdb4b4a/pdb
PDB entry 4b4a coloured by chain, front view.
PDB entry 4b4a coloured by chain, side view.
PDB entry 4b4a coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Structure of the TatC core of the twin-arginine protein transport system.
Rollauer SE, Tarry MJ, Graham JE, Jääskeläinen M, Jäger F, Johnson S, Krehenbrink M, Liu SM, Lukey MJ, Marcoux J, McDowell MA, Rodriguez F, Roversi P, Stansfeld PJ, Robinson CV, Sansom MS, Palmer T, Högbom M, Berks BC, Lea SM
Nature 492 210-4 (2012)
PMID: 23201679

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sec-independent protein translocase protein TatC Chain: A
Molecule details ›
Chain: A
Length: 249 amino acids
Theoretical weight: 28.42 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67305 (Residues: 1-240; Coverage: 100%)
Gene names: aq_1267, tatC
Sequence domains: Sec-independent protein translocase protein (TatC)

Ligands and Environments

1 bound ligand:
Ligand LMN 1 x LMN
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: R32
Unit cell:
a: 123.52Å b: 123.52Å c: 216.41Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.254 0.252 0.288
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Protein translocation across the inner membrane of Gram-negative bacteria: the Sec and Tat dependent protein transport pathways.
Kudva et al. (2013)
21 more

13 mentions without citation

Channel crossing: how are proteins shipped across the bacterial plasma membrane?
Collinson et al. (2015)
12 more