› 4b5k

X-ray diffraction
1.7Å resolution

Probing the active center of catalase-phenol oxidase from Scytalidium thermophilum

Released:
DOI: 10.2210/pdb4b5k/pdb
PDB entry 4b5k coloured by chain, front view.
PDB entry 4b5k coloured by chain, side view.
PDB entry 4b5k coloured by chain, top view.
Source organism: Mycothermus thermophilus
Primary publication:
Investigating the active centre of the Scytalidium thermophilum catalase.
Yuzugullu Y, Trinh CH, Fairhurst L, Ogel ZB, McPherson MJ, Pearson AR
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 369-75 (2013)
PMID: 23545640

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195736 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 719 amino acids
Theoretical weight: 79.28 KDa
Source organism: Mycothermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: R4GRT9 (Residues: 1-699; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HDD 4 x HDD
1 bound ligand:
Ligand CA 9 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: C2
Unit cell:
a: 201.392Å b: 122Å c: 125.67Å
α: 90° β: 115.48° γ: 90°
R-values:
R R work R free
0.154 0.153 0.18
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Not so monofunctional--a case of thermostable Thermobifida fusca catalase with peroxidase activity.
Lončar et al. (2015)
1 more

1 mention without citation

Investigating the active centre of the Scytalidium thermophilum catalase.
Yuzugullu et al. (2013)