› 4b6g

X-ray diffraction
1.4Å resolution

The Crystal Structure of the Neisserial Esterase D.

Released:
DOI: 10.2210/pdb4b6g/pdb
PDB entry 4b6g coloured by chain, front view.
PDB entry 4b6g coloured by chain, side view.
PDB entry 4b6g coloured by chain, top view.
Source organism: Neisseria meningitidis MC58
Primary publication:
A glutathione-dependent detoxification system is required for formaldehyde resistance and optimal survival of Neisseria meningitidis in biofilms.
Chen NH, Couñago RM, Djoko KY, Jennings MP, Apicella MA, Kobe B, McEwan AG
Antioxid Redox Signal 18 743-55 (2013)
PMID: 22937752

Function and Biology Details

Reaction catalysed: 
S-formylglutathione + H(2)O = glutathione + formate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-formylglutathione hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 32.39 KDa
Source organism: Neisseria meningitidis MC58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9JZ43 (Residues: 3-275; Coverage: 99%)
Gene name: NMB1305
Sequence domains: Putative esterase
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P6522
Unit cell:
a: 111.268Å b: 111.268Å c: 169.861Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.143 0.143 0.154
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Towards the sustainable discovery and development of new antibiotics.
Miethke et al. (2021)
8 more

7 mentions without citation

Fast and anisotropic flexibility-rigidity index for protein flexibility and fluctuation analysis.
Opron et al. (2014)
6 more