› 4b7m

X-ray diffraction
2.5Å resolution

H1N1 2009 Pandemic Influenza Virus: Resistance of the I223R Neuraminidase Mutant Explained by Kinetic and Structural Analysis

Released:
DOI: 10.2210/pdb4b7m/pdb
PDB entry 4b7m coloured by chain, front view.
PDB entry 4b7m coloured by chain, side view.
PDB entry 4b7m coloured by chain, top view.
Source organism: Influenza A virus (A/Netherlands/2631_1202/2010(H1N1))
Primary publication:
H1N1 2009 pandemic influenza virus: resistance of the I223R neuraminidase mutant explained by kinetic and structural analysis.
van der Vries E, Collins PJ, Vachieri SG, Xiong X, Liu J, Walker PA, Haire LF, Hay AJ, Schutten M, Osterhaus AD, Martin SR, Boucher CA, Skehel JJ, Gamblin SJ
PLoS Pathog 8 e1002914 (2012)
PMID: 23028314

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123733 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neuraminidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 469 amino acids
Theoretical weight: 51.7 KDa
Source organism: Influenza A virus (A/Netherlands/2631_1202/2010(H1N1))
Expression system: Gallus gallus
UniProt:
  • Canonical: F8UU09 (Residues: 1-469; Coverage: 100%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 3 x NAG
Ligand PO4 4 x PO4
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2221
Unit cell:
a: 118.579Å b: 162.474Å c: 118.941Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.205 0.237
Expression system: Gallus gallus

Quick links

Citations

2 review citations

Antiviral natural products and herbal medicines.
Lin et al. (2014)
1 more

5 mentions without citation

Structural characterization of a protective epitope spanning A(H1N1)pdm09 influenza virus neuraminidase monomers.
Wan et al. (2015)
4 more