› 4b7r

X-ray diffraction
1.9Å resolution

H1N1 2009 Pandemic Influenza Virus: Resistance of the I223R Neuraminidase Mutant Explained by Kinetic and Structural Analysis

Released:
DOI: 10.2210/pdb4b7r/pdb
PDB entry 4b7r coloured by chain, front view.
PDB entry 4b7r coloured by chain, side view.
PDB entry 4b7r coloured by chain, top view.
Source organism: Influenza A virus (A/California/07/2009(H1N1))
Primary publication:
H1N1 2009 pandemic influenza virus: resistance of the I223R neuraminidase mutant explained by kinetic and structural analysis.
van der Vries E, Collins PJ, Vachieri SG, Xiong X, Liu J, Walker PA, Haire LF, Hay AJ, Schutten M, Osterhaus AD, Martin SR, Boucher CA, Skehel JJ, Gamblin SJ
PLoS Pathog 8 e1002914 (2012)
PMID: 23028314

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111703 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Neuraminidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 387 amino acids
Theoretical weight: 42.64 KDa
Source organism: Influenza A virus (A/California/07/2009(H1N1))
UniProt:
  • Canonical: C7FH46 (Residues: 83-469; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand CA 8 x CA
Ligand G39 4 x G39
Ligand EPE 4 x EPE
Ligand NAG 12 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 82.65Å b: 148.82Å c: 166.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.173

Quick links

Citations

2 review citations

Antiviral natural products and herbal medicines.
Lin et al. (2014)
1 more

12 mentions without citation

H1N1 2009 pandemic influenza virus: resistance of the I223R neuraminidase mutant explained by kinetic and structural analysis.
van der Vries et al. (2012)
11 more