› 4b8l

X-ray diffraction
3Å resolution

Aurora B kinase P353G mutant

Released:
DOI: 10.2210/pdb4b8l/pdb
PDB entry 4b8l coloured by chain, front view.
PDB entry 4b8l coloured by chain, side view.
PDB entry 4b8l coloured by chain, top view.
Source organism: Xenopus laevis
Entry authors: Sessa F, Villa F

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126740 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aurora kinase B-A Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 33.6 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6DE08 (Residues: 78-361; Coverage: 79%)
Gene names: airk2-a, aurkb-a
Sequence domains: Protein kinase domain
Structure domains:
Inner centromere protein A Chain: D
Molecule details ›
Chain: D
Length: 44 amino acids
Theoretical weight: 5.07 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: O13024 (Residues: 797-840; Coverage: 5%)
Gene name: incenp-a
Sequence domains: Inner centromere protein, ARK binding region
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:
Ligand A0P 1 x A0P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 60.896Å b: 66.314Å c: 89.562Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.237 0.297
Expression system: Escherichia coli

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