› 4b8t

Solution NMR

RNA BINDING PROTEIN Solution structure of the third KH domain of KSRP in complex with the G-rich target sequence.

Released:
DOI: 10.2210/pdb4b8t/pdb
PDB entry 4b8t coloured by chain, ensemble of 20 models, front view.
PDB entry 4b8t coloured by chain, ensemble of 20 models, side view.
PDB entry 4b8t coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Noncanonical G recognition mediates KSRP regulation of let-7 biogenesis.
Nicastro G, García-Mayoral MF, Hollingworth D, Kelly G, Martin SR, Briata P, Gherzi R, Ramos A
Nat Struct Mol Biol 19 1282-6 (2012)
PMID: 23142982

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187818 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Far upstream element-binding protein 2 Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 10.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92945 (Residues: 317-418; Coverage: 14%)
Gene names: FUBP2, KHSRP
Sequence domains: KH domain
Structure domains: K Homology domain, type 1
5'-R(*AP*GP*GP*GP*UP)-3' Chain: B
Molecule details ›
Chain: B
Length: 5 nucleotides
Theoretical weight: 1.63 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 63%
Refinement method: ARIA
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

KH-RNA interactions: back in the groove.
Nicastro et al. (2015)
9 more

2 mentions without citation

Comprehensive Identification of RNA-Binding Domains in Human Cells.
Castello et al. (2016)
1 more