› 4b9a

X-ray diffraction
1.45Å resolution

Structure of a putative epoxide hydrolase from Pseudomonas aeruginosa.

Released:
DOI: 10.2210/pdb4b9a/pdb
PDB entry 4b9a coloured by chain, front view.
PDB entry 4b9a coloured by chain, side view.
PDB entry 4b9a coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
The AEROPATH project targeting Pseudomonas aeruginosa: crystallographic studies for assessment of potential targets in early-stage drug discovery.
Moynie L, Schnell R, McMahon SA, Sandalova T, Boulkerou WA, Schmidberger JW, Alphey M, Cukier C, Duthie F, Kopec J, Liu H, Jacewicz A, Hunter WN, Naismith JH, Schneider G
Acta Crystallogr Sect F Struct Biol Cryst Commun 69 25-34 (2013)
PMID: 23295481

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 33.62 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9I229 (Residues: 1-300; Coverage: 100%)
Gene name: PA2086
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand SO4 12 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P41212
Unit cell:
a: 83.91Å b: 83.91Å c: 140.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.112 0.111 0.138
Expression system: Escherichia coli BL21

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Citations

4 review citations

Lytic transglycosylases: concinnity in concision of the bacterial cell wall.
Dik et al. (2017)
3 more

6 mentions without citation

Gapless quantum spin liquid ground state in the two-dimensional spin-1/2 triangular antiferromagnet YbMgGaO4.
Li et al. (2015)
5 more