› 4bab

X-ray diffraction
2.56Å resolution

Redesign of a Phenylalanine Aminomutase into a beta-Phenylalanine Ammonia Lyase

Released:
DOI: 10.2210/pdb4bab/pdb
PDB entry 4bab coloured by chain, front view.
PDB entry 4bab coloured by chain, side view.
PDB entry 4bab coloured by chain, top view.
Source organism: Taxus chinensis
Entry authors: Bartsch S, Wybenga GG, Jansen M, Heberling MM, Wu B, Dijkstra BW, Janssen DB

Function and Biology Details

Reactions catalysed: 
L-phenylalanine = trans-cinnamate + ammonia
L-phenylalanine = L-beta-phenylalanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179344 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine aminomutase (L-beta-phenylalanine forming) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 705 amino acids
Theoretical weight: 77.55 KDa
Source organism: Taxus chinensis
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q68G84 (Residues: 1-687; Coverage: 100%)
Gene name: pam
Sequence domains: Aromatic amino acid lyase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MDO 4 x MDO

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 94.136Å b: 132.095Å c: 110.035Å
α: 90° β: 95.52° γ: 90°
R-values:
R R work R free
0.216 0.214 0.251
Expression system: Escherichia coli K-12

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