› 4bbj

X-ray diffraction
2.75Å resolution

Copper-transporting PIB-ATPase in complex with beryllium fluoride representing the E2P state

Released:
DOI: 10.2210/pdb4bbj/pdb
Model geometry
Fit model/data
PDB entry 4bbj coloured by chain, front view.
PDB entry 4bbj coloured by chain, side view.
PDB entry 4bbj coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila
Primary publication:
Copper-transporting P-type ATPases use a unique ion-release pathway.
Andersson M, Mattle D, Sitsel O, Klymchuk T, Nielsen AM, Møller LB, White SH, Nissen P, Gourdon P
Nat Struct Mol Biol 21 43-8 (2014)
PMID: 24317491

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Cu(+)(Side 1) = ADP + phosphate + Cu(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178686 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-exporting P-type ATPase Chain: A
Molecule details ›
Chain: A
Length: 736 amino acids
Theoretical weight: 78.4 KDa
Source organism: Legionella pneumophila subsp. pneumophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5ZWR1 (Residues: 1-736; Coverage: 100%)
Gene names: copA, lpg1024
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand NAD 1 x NAD
Ligand CE1 1 x CE1
Ligand PC 1 x PC
1 modified residue:
Ligand BFD 1 x BFD

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 242.01Å b: 71.37Å c: 72.43Å
α: 90° β: 100.01° γ: 90°
R-values:
R R work R free
0.202 0.199 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Copper tolerance and virulence in bacteria.
Ladomersky et al. (2015)
16 more

14 mentions without citation

The Role of Copper Chaperone Atox1 in Coupling Redox Homeostasis to Intracellular Copper Distribution.
Hatori et al. (2016)
13 more