› 4bem

X-ray diffraction
2.1Å resolution

Crystal structure of the F-type ATP synthase c-ring from Acetobacterium woodii.

Released:
DOI: 10.2210/pdb4bem/pdb
PDB entry 4bem coloured by chain, front view.
PDB entry 4bem coloured by chain, side view.
PDB entry 4bem coloured by chain, top view.
Source organism: Acetobacterium woodii DSM 1030
Primary publication:
High-resolution structure and mechanism of an F/V-hybrid rotor ring in a Na⁺-coupled ATP synthase.
Matthies D, Zhou W, Klyszejko AL, Anselmi C, Yildiz Ö, Brandt K, Müller V, Faraldo-Gómez JD, Meier T
Nat Commun 5 5286 (2014)
PMID: 25381992

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124808 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP synthase subunit c Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 82 amino acids
Theoretical weight: 8.21 KDa
Source organism: Acetobacterium woodii DSM 1030
UniProt:
  • Canonical: H6LFT2 (Residues: 1-82; Coverage: 100%)
Gene names: Awo_c02170, Awo_c02180, atpE, atpE2, atpE3
Sequence domains: ATP synthase subunit C
Structure domains: F1F0 ATP synthase subunit C
ATP synthase subunit c Chain: J
Molecule details ›
Chain: J
Length: 182 amino acids
Theoretical weight: 18.41 KDa
Source organism: Acetobacterium woodii DSM 1030
UniProt:
  • Canonical: H6LFT0 (Residues: 1-182; Coverage: 100%)
Gene names: Awo_c02160, atpE, atpE1
Sequence domains: ATP synthase subunit C
Structure domains: lithium bound rotor ring of v- atpase

Ligands and Environments

6 bound ligands:
Ligand NA 10 x NA
Ligand HTG 19 x HTG
Ligand MN 1 x MN
Ligand TAM 1 x TAM
Ligand P6G 1 x P6G
Ligand ACT 4 x ACT
1 modified residue:
Ligand FME 10 x FME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P43212
Unit cell:
a: 121.17Å b: 121.17Å c: 150.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.22

Quick links

Citations

7 review citations

Energetics and Application of Heterotrophy in Acetogenic Bacteria.
Schuchmann et al. (2016)
6 more

4 mentions without citation

Membrane-spanning α-helical barrels as tractable protein-design targets.
Niitsu et al. (2017)
3 more