› 4bep

X-ray diffraction
3.14Å resolution

Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein (apo-form)

Released:
DOI: 10.2210/pdb4bep/pdb
PDB entry 4bep coloured by chain, front view.
PDB entry 4bep coloured by chain, side view.
PDB entry 4bep coloured by chain, top view.
Source organism: Legionella pneumophila
Primary publication:
Structure of the Legionella effector AnkX reveals the mechanism of phosphocholine transfer by the FIC domain.
Campanacci V, Mukherjee S, Roy CR, Cherfils J
EMBO J 32 1469-77 (2013)
PMID: 23572077

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178710 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphocholine transferase AnkX Chains: A, B
Molecule details ›
Chains: A, B
Length: 512 amino acids
Theoretical weight: 58.91 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5ZXN6 (Residues: 2-484; Coverage: 51%)
Gene names: ankX, legA8, lpg0695
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 55.268Å b: 91.633Å c: 239.151Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.286
Expression system: Escherichia coli BL21(DE3)

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Citations

22 review citations

Review series: Rab GTPases and membrane identity: causal or inconsequential?
Barr FA. (2013)
21 more

3 mentions without citation

The unity of opposites: Strategic interplay between bacterial effectors to regulate cellular homeostasis.
Iyer et al. (2021)
2 more