› 4bf5

X-ray diffraction
1.45Å resolution

Structure of broad spectrum racemase from Aeromonas hydrophila

Released:
DOI: 10.2210/pdb4bf5/pdb
Model geometry
Fit model/data
PDB entry 4bf5 coloured by chain, front view.
PDB entry 4bf5 coloured by chain, side view.
PDB entry 4bf5 coloured by chain, top view.
Source organism: Aeromonas hydrophila subsp. hydrophila
Primary publication:
Structural basis for the broad specificity of a new family of amino-acid racemases.
Espaillat A, Carrasco-López C, Bernardo-García N, Pietrosemoli N, Otero LH, Álvarez L, de Pedro MA, Pazos F, Davis BM, Waldor MK, Hermoso JA, Cava F
Acta Crystallogr D Biol Crystallogr 70 79-90 (2014)
PMID: 24419381

Function and Biology Details

Reaction catalysed: 
An L-amino acid = a D-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Broad specificity amino-acid racemase Chains: A, B
Molecule details ›
Chains: A, B
Length: 417 amino acids
Theoretical weight: 45.69 KDa
Source organism: Aeromonas hydrophila subsp. hydrophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0KLG5 (Residues: 1-408; Coverage: 100%)
Gene names: AHA_2607, alr-3, bsr
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
3 bound ligands:
Ligand CL 23 x CL
Ligand GOL 7 x GOL
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 56.666Å b: 81.85Å c: 78.286Å
α: 90° β: 98.97° γ: 90°
R-values:
R R work R free
0.193 0.192 0.208
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Bacterial synthesis of D-amino acids.
Radkov et al. (2014)
11 more

1 mention without citation

Structural basis for the broad specificity of a new family of amino-acid racemases.
Espaillat et al. (2014)