› 4bfq

X-ray diffraction
2.4Å resolution

Assembly of a triple pi-stack of ligands in the binding site of Aplysia californica acetylcholine binding protein (AChBP)

Released:
DOI: 10.2210/pdb4bfq/pdb
PDB entry 4bfq coloured by chain, front view.
PDB entry 4bfq coloured by chain, side view.
PDB entry 4bfq coloured by chain, top view.
Source organism: Aplysia californica
Primary publication:
Assembly of a π-π stack of ligands in the binding site of an acetylcholine-binding protein.
Stornaiuolo M, De Kloe GE, Rucktooa P, Fish A, van Elk R, Edink ES, Bertrand D, Smit AB, de Esch IJ, Sixma TK
Nat Commun 4 1875 (2013)
PMID: 23695669

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurotransmitter-gated ion-channel ligand-binding domain-containing protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 217 amino acids
Theoretical weight: 24.69 KDa
Source organism: Aplysia californica
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8WSF8 (Residues: 20-236; Coverage: 100%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

2 bound ligands:
Ligand 083 14 x 083
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 80.73Å b: 78.33Å c: 106.53Å
α: 90° β: 102.67° γ: 90°
R-values:
R R work R free
0.212 0.21 0.247
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Principles of agonist recognition in Cys-loop receptors.
Lynagh et al. (2014)
3 more

4 mentions without citation

Structural Studies of GABAA Receptor Binding Sites: Which Experimental Structure Tells us What?
Puthenkalam et al. (2016)
3 more