› 4bgd

X-ray diffraction
3.1Å resolution

Crystal structure of Brr2 in complex with the Jab1/MPN domain of Prp8

Released:
DOI: 10.2210/pdb4bgd/pdb
PDB entry 4bgd coloured by chain, front view.
PDB entry 4bgd coloured by chain, side view.
PDB entry 4bgd coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis of Brr2-Prp8 interactions and implications for U5 snRNP biogenesis and the spliceosome active site.
Nguyen TH, Li J, Galej WP, Oshikane H, Newman AJ, Nagai K
Structure 21 910-19 (2013)
PMID: 23727230

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152414 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pre-mRNA-splicing helicase BRR2 Chain: A
Molecule details ›
Chain: A
Length: 1722 amino acids
Theoretical weight: 195.73 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32639 (Residues: 442-2163; Coverage: 80%)
Gene names: BRR2, RSS1, SNU246, SYGP-ORF66, YER172C
Sequence domains:
Structure domains:
Pre-mRNA-splicing factor 8 Chain: C
Molecule details ›
Chain: C
Length: 248 amino acids
Theoretical weight: 28.18 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P33334 (Residues: 2148-2395; Coverage: 10%)
Gene names: DBF3, DNA39, PRP8, RNA8, SLT21, USA2, YHR165C
Sequence domains: PROCT (NUC072) domain
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
Ligand PE5 1 x PE5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 107.64Å b: 178.64Å c: 180.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.27
Expression system: Saccharomyces cerevisiae

Quick links

Citations

16 review citations

Mechanistic insights into precursor messenger RNA splicing by the spliceosome.
Shi Y. (2017)
15 more

11 mentions without citation

Structural studies of the spliceosome: zooming into the heart of the machine.
Galej et al. (2014)
10 more