› 4bgp

X-ray diffraction
1.8Å resolution

Crystal structure of La Crosse virus nucleoprotein

Released:
DOI: 10.2210/pdb4bgp/pdb
PDB entry 4bgp coloured by chain, front view.
PDB entry 4bgp coloured by chain, side view.
PDB entry 4bgp coloured by chain, top view.
Source organism: La Crosse virus
Primary publication:
Structural basis for encapsidation of genomic RNA by La Crosse Orthobunyavirus nucleoprotein.
Reguera J, Malet H, Weber F, Cusack S
Proc Natl Acad Sci U S A 110 7246-51 (2013)
PMID: 23589854

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138221 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleoprotein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 26.7 KDa
Source organism: La Crosse virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04873 (Residues: 1-235; Coverage: 100%)
Gene name: N
Sequence domains: Bunyavirus nucleocapsid (N) protein
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand SO4 2 x SO4
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 62.59Å b: 70.08Å c: 70.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.204
Expression system: Escherichia coli BL21(DE3)

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Citations

19 review citations

Orthobunyaviruses: recent genetic and structural insights.
Elliott RM. (2014)
18 more

4 mentions without citation

Structural basis for encapsidation of genomic RNA by La Crosse Orthobunyavirus nucleoprotein.
Reguera et al. (2013)
3 more